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Anti-BrdU (Bu20a) antibody

SKU Product Brand Unit Availability Price Quantity  
AB-06-0559
Anti-BrdU (Bu20a) antibody
Erpan Tech In stock

Specifications        

Product type: Primary antibody
Antigen: BrdU (Bu20a)
Immunogen: Raised against bromodeoxyuridine
Species immunized: Mouse
Isotype: IgG1
Applications: Immunofluorescence (1:100-500); Immunohistochemistry-Paraffin (1:100-500); Flow Cytometry (1ug/test)
Reactivity: Bromodeoxyuridine
Clonality (clone number): Monoclonal, 1G1
Form: Liquid
Buffer: Tris-HCl buffer (pH7.4), 1% BSA, 40% glycerol, 0.05% NaN3.
Concentration: 1mg/ml
Purity: Protein A/G affinity purified
Storage: Aliquot and freeze at -20℃. Avoid multiple freeze/thaw cycles.
Alternative names: Bromodeoxyuridine antibody
BUdr antibody
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Target information

The halogenated pyrimidine thymidine analog bromodeo-xyuridine (BrdU) is incorporated into newly synthesized DNA strands of S-phase cells and is useful for estimating the fraction of cells in S-phase. Additionally, the analysis of the uptake of BrdU is a reliable method to quantitate the degree of DNA-synthesis. BrdU is also useful for studying sister chromatid exchange and to isolate nascent DNA. UV-induced excision-repair synthesis is one method for incorporating BrdU into cellular DNA. Anti-BrdU antibodies bind to the exposed BrdU in single-stranded DNA after a hydrochloric acid denaturation step or nuclease digestion. Protease antigen recovery is necessary for most tissues or cells fixed with crosslinking agents such as formalin but may decrease the specificity of BrdUrd immunodetection. The monoclonal antibody Bu20a against BrdU stains BrdU incorporated into the nulcei of a wide range of proliferating cell types including human tumors growing in nude mice and tonsil lymphoid.

Provider

Erpantech Laboratory

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