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Anti-Brd4 antibody

SKU Product Brand Unit Availability Price Quantity  
AB-06-0557
Anti-Brd4 antibody
Erpan Tech In stock

Specifications        

Product type: Primary antibody
Antigen: Brd4
Immunogen: Recombinant protein within Human Brd4 aa 1-120
Species immunized: Rabbit
Isotype: IgG
Applications: Western Blot (1:500-1:1000); Immunocytochemistry (1:100-1:200); Immunohistochemistry (1:100-1:200)
Reactivity: Mouse, Human
Clonality (clone number): Polyclonal
Form: Liquid
Buffer: Tris-HCl buffer (pH7.4), 1% BSA, 50% glycerol, 0.05% NaN3.
Concentration: 1mg/ml
Purity: Protein A/G affinity purified
Storage: Aliquot and freeze at -20℃. Avoid multiple freeze/thaw cycles.
Alternative names: Brd4 antibody
BRD4-NUT FUSION antibody
BRD4-NUT fusion oncoprotein antibody
BRD4_HUMAN antibody
Bromodomain containing 4 antibody
Bromodomain containing protein 4 antibody
Bromodomain-containing protein 4 antibody
CAP antibody
Chromosome associated protein antibody
HUNK1 antibody
HUNKI antibody
MCAP antibody
Mitotic chromosome-associated protein antibody
Show More

Target information

Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure (PubMed:23589332, PubMed:23317504, PubMed:22334664). During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters. Also recruits P-TEFb complex to distal enhancers, so called anti-pause enhancers in collaboration with JMJD6. BRD4 and JMJD6 are required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II (PubMed:23589332, PubMed:19596240, PubMed:16109377, PubMed:16109376, PubMed:24360279). Promotes phosphorylation of ‘Ser-2’ of the C-terminal domain (CTD) of RNA polymerase II (PubMed:23086925). According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of ‘Ser-2’ of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo (PubMed:22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B (PubMed:19103749). Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters (PubMed:23317504).

Provider

Erpantech Laboratory

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