Anti-ALKBH5 Recombinant Rabbit Monoclonal antibody
Specifications
| Product Cat#: | AB-06-5095 |
| Product type: | Primary antibody |
| Antigen: | WDR41 |
| Immunogen: | Recombinant protein within human ALKBH5 aa 51-350/394. |
| Predicted molecular weight: | 44 kDa |
| Observed molecular weight: | 35-44 kDa |
| Species immunized: | Rabbit |
| Isotype: | IgG |
| Applications: | Western Blot (1:500-1:2000); Immunohistochemistry-Paraffin (1:500-1:2000) |
| Reactivity: | Human, Rat, Mouse |
| Clonality (clone number): | Monoclonal, PSH01-16 |
| Form: | Liquid |
| Buffer: | 1*PBS buffer (pH 7.4), 0.1% BSA, 40% Glycerol, 0.05% NaN3. |
| Concentration: | 1 mg/mL |
| Purity: | Protein A affinity purified. |
| Storage: | Aliquot and freeze at -20℃. Avoid multiple freeze/thaw cycles. |
| Alternative names: | ABH5 antibody |
| AlkB, alkylation repair homolog 5 (E. coli) antibody | |
| AlkB, alkylation repair homolog 5 antibody | |
| Alkylated DNA repair protein alkB homolog 5 antibody | |
| Alpha ketoglutarate dependent dioxygenase alkB homolog 5 antibody | |
| OFOXD antibody | |
| OFOXD1 antibody | |
| Oxoglutarate and iron-dependent oxygenase domain containing antibody | |
| Probable alpha ketoglutarate dependent dioxygenase ABH5 antibody | |
| RNA demethylase ALKBH5 antibody |
Target information
Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N6-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24489119, PubMed:24616105, PubMed:24778178).
Can also demethylate N6-methyladenosine in single-stranded DNA (in vitro) (PubMed:24616105).
Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24489119, PubMed:24616105, PubMed:24778178).
Demethylation of m6A mRNA affects mRNA processing and export (PubMed:23177736).
Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3′-UTR mRNAs in male germ cells (By similarity).
Provider
Erpantech Laboratory