Anti-AAK1 antibody
Specifications
| Product Cat#: | AB-07-2257 |
| Product type: | Primary antibody |
| Antigen: | AAK1 |
| Immunogen: | The antiserum was produced against synthesized peptide derived from internal of human AAK1. |
| Species immunized: | Rabbit |
| Isotype: | IgG |
| Applications: | Western Blot (1:500-1:750); ELISA (1:15000) |
| Reactivity: | Human, Mouse |
| Clonality (clone number): | Polyclonal |
| Form: | Liquid |
| Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Concentration: | 1 mg/ml |
| Purity: | Antigen affinity chromatography |
| Storage: | Aliquot and freeze at -20°C. Avoid multiple freeze/thaw cycles. |
| Alternative names: | AP2-associated protein kinase 1, EC 2.7.11.1, Adaptor-associated kinase 1, AAK1, KIAA1048 |
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Target information
Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.
Provider
Erpantech Laboratory
