Anti-Phospho-IRF3 (S386) antibody
Specifications
| Product Cat#: | AB-06-3507 |
| Product type: | Primary antibody |
| Antigen: | Phospho-IRF3(S386) |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser386 of human IRF3. |
| Species immunized: | Rabbit |
| Isotype: | IgG |
| Applications: | Western Blot (1:900); Immunocytochemistry (1:40-1:200); Immunofluorescence |
| Reactivity: | Human |
| Clonality (clone number): | Monoclonal (SU03-28) |
| Form: | Liquid |
| Buffer: | Tris-HCl buffer (pH7.4), 1% BSA; 40% glycerol, 0.05% NaN3. |
| Concentration: | 1 mg/ml |
| Purity: | Protein A affinity purified |
| Storage: | Aliquot and freeze at -20℃. Avoid multiple freeze/thaw cycles. |
| Alternative names: | IIAE7 antibody |
| Interferon regulatory factor 3 antibody | |
| IRF 3 antibody | |
| IRF-3 antibody | |
| IRF3 antibody | |
| IRF3_HUMAN antibody | |
| MGC94729 antibody |
Target information
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses . Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters . Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction . Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages .
Activity regulationi
Provider
Erpantech Laboratory
