Anti-PMS2 antibody
Specifications
| Product Cat#: | AB-06-3630 |
| Product type: | Primary antibody |
| Antigen: | PMS2 |
| Immunogen: | Recombinant protein |
| Species immunized: | Rabbit |
| Isotype: | IgG |
| Applications: | Western Blot (1:500-1:2000); Immunohistochemistry (1:50-1:200); Immunocytochemistry (1:50-1:200); Flow Cytometry (1:50-1:100); Immunofluorescence; Immunoprecipitation |
| Reactivity: | Human |
| Clonality (clone number): | Monoclonal (SY08-09) |
| Form: | Liquid |
| Buffer: | Tris-HCl buffer (pH7.4), 1% BSA, 40% Glycerol, 0.05% NaN3. |
| Concentration: | 1 mg/ml |
| Purity: | Protein A affinity purified |
| Storage: | Aliquot and freeze at -20℃. Avoid multiple freeze/thaw cycles. |
| Alternative names: | DNA Mismatch Repair Gene Homologue antibody |
| DNA Mismatch Repair Protein PMS2 antibody | |
| H_DJ0042M02.9 antibody | |
| HNPCC4 antibody | |
| Mismatch Repair Endonuclease PMS2 antibody | |
| Mismatch Repair Gene PMSL2 antibody | |
| PMS 2 antibody | |
| PMS1 Protein Homolog 2 antibody | |
| PMS2 antibody | |
| PMS2 Postmeiotic Segregation Increased 2 antibody | |
| PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) antibody | |
| PMS2_HUMAN antibody | |
| PMS2CL antibody | |
| PMSL2 antibody |
Target information
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.
Provider
Erpantech Laboratory
