Anti-EGFR antibody (KO Validated)
Specifications
| Product Cat#: | AB-06-4830 |
| Product type: | Primary antibody |
| Antigen: | EGFR |
| Immunogen: | Recombinant protein within human EGFR aa 900-1150. |
| Species immunized: | Mouse |
| Isotype: | IgG2b |
| Applications: | Western Blot (1:250-1:1000); Immunohistochemistry-Paraffin (1:100-200); Immunocytochemistry (1:25-1:100); Flow Cytometry (1:25-1:100) |
| Reactivity: | Human |
| Clonality (clone number): | Monoclonal; [7-F2-F8] |
| Form: | Liquid |
| Buffer: | 1*PBS buffer (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1 mg/ml |
| Purity: | Protein A affinity purified. |
| Storage: | Aliquot and freeze at -20°C. Avoid multiple freeze/thaw cycles. |
| Conjugate: | Non-conjugated |
| Alternative names: | Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody |
| Cell growth inhibiting protein 40 antibody | |
| Cell proliferation inducing protein 61 antibody | |
| EGF R antibody | |
| EGFR antibody | |
| EGFR_HUMAN antibody | |
| Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody | |
| Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody | |
| Epidermal growth factor receptor antibody | |
| erb-b2 receptor tyrosine kinase 1 antibody | |
| ERBB antibody | |
| ERBB1 antibody | |
| Errp antibody | |
| HER1 antibody | |
| mENA antibody | |
| NISBD2 antibody | |
| Oncogen ERBB antibody | |
| PIG61 antibody | |
| Proto-oncogene c-ErbB-1 antibody | |
| Receptor tyrosine protein kinase ErbB 1 antibody | |
| Receptor tyrosine-protein kinase ErbB-1 antibody | |
| SA7 antibody | |
| Species antigen 7 antibody | |
| Urogastrone antibody | |
| v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody | |
| wa2 antibody | |
| Wa5 antibody |
Target information
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:2790960, PubMed:10805725, PubMed:27153536).
Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975, PubMed:15611079, PubMed:12297049, PubMed:27153536, PubMed:20837704, PubMed:17909029).
Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536).
May also activate the NF-kappa-B signaling cascade (PubMed:11116146).
Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed:11602604).
Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed:11483589).
Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed:20462955).
Plays a role in enhancing learning and memory performance (By similarity).
Provider
Erpantech Laboratory
