Anti-RIP3 antibody (KO Validated)
Specifications
| Product Cat#: | AB-06-4827 |
| Product type: | Primary antibody |
| Antigen: | RIP3 |
| Immunogen: | Synthetic peptide within Mouse RIP3 aa 440-486/486. |
| Species immunized: | Rabbit |
| Isotype: | IgG |
| Applications: | Western Blot (1:250-1:1000) |
| Reactivity: | Mouse, Rat |
| Clonality (clone number): | Polyclonal |
| Form: | Liquid |
| Buffer: | 1*PBS buffer (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1 mg/ml |
| Purity: | Immunogen affinity purified. |
| Storage: | Aliquot and freeze at -20°C. Avoid multiple freeze/thaw cycles. |
| Conjugate: | Non-conjugated |
| Alternative names: | Receptor interacting protein 3 antibody |
| Receptor interacting serine threonine kinase 3 antibody | |
| Receptor interacting serine/threonine protein kinase 3 antibody | |
| Receptor-interacting protein 3 antibody | |
| Receptor-interacting serine/threonine-protein kinase 3 antibody | |
| RIP 3 antibody | |
| RIP like protein kinase 3 antibody | |
| RIP-3 antibody | |
| RIP-like protein kinase 3 antibody | |
| RIPK 3 antibody | |
| RIPK3 antibody | |
| RIPK3_HUMAN antibody |
Target information
Serine/threonine-protein kinase that activates necroptosis and apoptosis, two parallel forms of cell death (PubMed:27321907, PubMed:27746097, PubMed:27917412, PubMed:28607035, PubMed:32200799, PubMed:32296175).
Necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members, is triggered by RIPK3 following activation by ZBP1 (PubMed:19590578, PubMed:22423968, PubMed:24012422, PubMed:24019532, PubMed:24557836, PubMed:27746097, PubMed:27819681, PubMed:27819682, PubMed:24095729, PubMed:32200799, PubMed:27321907, PubMed:32296175).
Activated RIPK3 forms a necrosis-inducing complex and mediates phosphorylation of MLKL, promoting MLKL localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed:24813849, PubMed:24813850, PubMed:27321907).
In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol (PubMed:32200799, PubMed:32296175).
Also regulates apoptosis: apoptosis depends on RIPK1, FADD and CASP8, and is independent of MLKL and RIPK3 kinase activity (PubMed:27321907).
Phosphorylates RIPK1: RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation (By similarity).
In some cell types, also able to restrict viral replication by promoting cell death-independent responses (PubMed:30635240).
In response to flavivirus infection in neurons, promotes a cell death-independent pathway that restricts viral replication: together with ZBP1, promotes a death-independent transcriptional program that modifies the cellular metabolism via up-regulation expression of the enzyme ACOD1/IRG1 and production of the metabolite itaconate (PubMed:30635240).
Itaconate inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes (PubMed:30635240).
RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL (By similarity).
These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production (By similarity).
Provider
Erpantech Laboratory
