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Anti-PCNA antibody

SKU Product Brand Unit Availability Price Quantity  
AB-07-3444
Anti-PCNA antibody
Erpan Tech In stock

Specifications        

Product Cat#: AB-07-3444
Product type: Primary antibody
Antigen: PCNA
Immunogen: A synthetic peptide derived from human PCNA. The exact amino-acid sequence is proprietary.
Species immunized: Rabbit
Isotype: IgG
Applications: ELISA (1:4500); Western Blot (1:500-1:2500); Immunohistochemistry (1:50-1:100)
Reactivity: Human, Mouse, Rat
Clonality (clone number): Polyclonal
Form: Liquid
Buffer: PBS (without Mg2+ and Ca2+ ), pH 7.4, 0.02% sodium azide and 50% glycerol
Concentration: 1 mg/ml
Purity: Antigen affinity chromatography
Storage: Aliquot and freeze at -20°C. Avoid multiple freeze/thaw cycles.
Alternative names: Cyclin, Proliferating cell nuclear antigen
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Target information

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase’s processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3′-5′ exonuclease and 3′-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while ‘Lys-63’-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737).

Provider

Erpantech Laboratory

download

MSDS-AB-07-3444.pdf (143 downloads)